Cytotoxic and Antioxidant Activity of a Chemically Characterized Extract of Smilax aspera Leaves and Stems

The present study identifies the phytochemical profile of a hydroalcoholic extract derived from Smilax aspera leaves and stems, estimates its antioxidant capacity evaluates cytotoxic activity against glioblastoma (A172 cell line) rhabdomyosarcoma (TE671 line). Chemical analysis stems was performed with liquid chromatography combined quadrupole time-of-flight high-resolution mass spectrometry (LC/Q-TOF/HRMS). evaluated 2,2-Diphenyl-1-picrylhydrazyl (DPPH) 2,2′-azinobis[3-ethylbenzthiazoline-6-acid)] (ABTS) assays. Cell viability examined using alamar blue assay. Most compounds tentatively identified belonged to flavonoids family, rutin being most abundant, followed by luteolin. showed potent which corresponded 13.9 ± 1.91 μg/mL (DPPH assay) 6.27 1.7 (ABTS assay), expressed as IC50 values. inhibited proliferation cancer cells. lowest value for A172 cells observed 48 h after treatment calculated at 0.482 0.98 mg/mL while TE671 line 0.629 1.31 mg/mL, 72 treatment. Considering high biological health defense promoters, S. can be an important natural source consider they may provide benefits.